Guidance^® UTI Publications.

1

Peer-reviewed

Full Validation of Pooled Antibiotic Susceptibility Testing Using CLSI Methods and Performance Criteria in UTI Pathogens

Antibiotics · 20 November 2025

Richard A. Festa, Frank R. Cockerill, Rick L. Pesano, Emery Haley, Natalie Luke, Mohit Mathur, et al.

View on Journal Site

2

Peer-reviewed

Improving Patient Outcomes While Reducing Empirical Treatment with Multiplex-Polymerase-Chain-Reaction/Pooled-Antibiotic-Susceptibility-Testing Assay for Complicated and Recurrent Urinary Tract Infections

MDPI · 26 September 2023

Emery Haley, Natalie Luke, Howard Korman, David Baunoch, Dakun Wang, Xinhua Zhao and Mohit Mathur.

Read summary

3

Peer-reviewed

Real-World Evidence That a Novel Diagnostic Combining Molecular Testing With Pooled Antibiotic Susceptibility Testing is Associated With Reduced Infection Severity and Lower Cost Compared With Standard Urine Culture in Patients With Complicated or Persistently Recurrent Urinary Tract Infections

JU Open Plus · 23 May 2023

Dicken Ko, Emily Lukacz, Iver Allen Juster, Timothy Niecko, Aparna Ashok, Jean Annah Vollstedt, David Baunoch, Mohit Mathur.

Read summary

4

Peer-reviewed

Diagnostic Test Combining Molecular Testing with Phenotypic Pooled Antibiotic Susceptibility Improved the Clinical Outcomes of Patients with Non-E. coli or Polymicrobial Complicated Urinary Tract Infections

Research and Reports in Urology · 1 May 2023

Howard J Korman, David Baunoch, Natalie Luke, Dakun Wang, Xihua Zhao, Michael Levin, David L Wenzler, Mohit Mathur.

Read summary

5

Peer-reviewed

Multiplex Polymerase Chain Reaction/Pooled Antibiotic Susceptibility Testing Was Not Associated with Increased Antibiotic Resistance in Management of Complicated Urinary Tract Infections

Infection and Drug Resistance · 9 May 2023

Howard J Korman, Mohit Mathur, Natalie Luke, Dakun Wang, Xihua Zhao, Michael Levin, David L Wenzler, David Baunoch.

Read summary

6

Peer-reviewed

Utilization of M-PCR and P-AST for Diagnosis and Management of Urinary Tract Infections in Home-Based Primary Care

JOJ Urology & Nephrology · 27 March 2020

Annemarie Daly, David Baunoch, Kelly Rehling, Natalie Luke, Meghan Campbell, Patrick Cacdac, Miguel Penaranda, Michael Opel, Shuguang Huang, Xinhua Zhao and Kirk Wojno.

View on Journal Site

7

Peer-reviewed

The Diagnosis of Urinary Tract Infections Using a Novel At-home Testing Protocol to Enhance Telemedicine: A Retrospective Analysis

Ambulatory, Office-based, and Geriatric Urology · 9 January 2023

Andrew Korman, Siddharth Ramanathan, Nathan Shen, Zachary A. Gerndt, Natalie Luke, Dakun Wang, Hannah Zhao, Shuguang Huang, Rajan Dewar, Kirk Wojno, Larry Sirls, Savitha Balaraman, and Howard Korman.

Read summary

8

Peer-reviewed

Bacterial Interactions as Detected by Pooled Antibiotic Susceptibility Testing (P-AST) in Polymicrobial Urine Specimens

Journal of Surgical Urology · 29 May 2020

Annah Vollstedt,  David Baunoch,  Alan Wolfe,  Natalie Luke,  Kirk J Wojno,  Kevin Cline, et al.

View on Journal Site

9

Peer-reviewed

Concordance Between Antibiotic Resistance Genes and Susceptibility in Symptomatic Urinary Tract Infections.

Infection and Drug Resistance · 19 August 2021

David Baunoch, Natalie Luke, Dakun Wang, Annah Vollstedt, Xinhua Zhao, Dicken S C Ko, Shuguang Huang, Patrick Cacdac, and Larry T Sirls.

View on Journal Site

10

Peer-reviewed

Pooled Antibiotic Susceptibility Testing Performs Within CLSI Standards for Validation When Measured Against Broth Microdilution and Disk Diffusion Antibiotic Susceptibility Testing of Cultured Isolates

Antibiotics · 14 December 2024

Emery Haley, Frank R. Cockerill, Rick L. Pesano, Richard A. Festa, Natalie Luke, Mohit Mathur, Xiaofei Chen, Jim Havrilla, and David Baunoch.

View on Journal Site

11

Peer-reviewed

Pooled Antibiotic Susceptibility Testing for Polymicrobial UTI Performs Within CLSI Validation Standards

Antibiotics · 1 February 2025

Richard A. Festa, Frank R. Cockerill, Rick L. Pesano, Emery Haley, Natalie Luke, Mohit Mathur, et al.

View on Journal Site

12

Peer-reviewed

Noninferiority of Multiplex Polymerase Chain Reaction Compared to Standard Urine Culture for Urinary Tract Infection Diagnosis in Pediatric Patients at Hackensack Meridian Health Children’s Hospital Emergency Department

Pediatric Health Med Ther · 22 November 2024

Sejal M Bhavsar, Nisha Polavarapu, Emery Haley, Natalie Luke, Mohit Mathur, Xiaofei Chen, Jim Havrilla, David Baunoch, and Kenneth Lieberman

View on Journal Site

13

Peer-reviewed

Urine Biomarkers Individually and as a Consensus Model Show High Sensitivity and Specificity for Detecting UTIs

BMC Infectious Diseases · 31 January 2024

Marzieh Akhlaghpour,  Emery Haley,  Laura Parnell,  Natalie Luke,  Mohit Mathur,  Richard A Festa, et al.

View on Journal Site

14

Peer-reviewed

Multiplex PCR Based Urinary Tract Infection (UTI) Analysis Compared to Traditional Urine Culture in Identifying Significant Pathogens in Symptomatic Patients

Urology · 9 November 2019

Kirk J Wojno,  David Baunoch,  Natalie Luke,  Michael Opel,  Howard Korman,  Colleen Kelly, et al.

View on Journal Site

15

Peer-reviewed

Multisite Prospective Comparison of Multiplex Polymerase Chain Reaction Testing with Urine Culture for Diagnosis of Urinary Tract Infections in Symptomatic Patients

Journal of Surgical Urology · 13 July 2020

Annah Vollstedt, David Baunoch, Kirk J. Wojno, Natalie Luke, Kevin Cline, Laurence Belkoff, et al.

View on Journal Site

16

Peer-reviewed

Quantitative multiplex polymerase chain reaction in copies ml–1 linearly correlates with standard urine culture in colonies ml−1 for urinary tract infection (UTI) pathogens

Letters in Applied Microbiology · 27 July 2023

Richard A Festa, Mike Opel, Mohit Mathur, Natalie Luke, Laura K S Parnell, Dakun Wang, Xinhua Zhao, Jesus Magallon, Michael Percaccio, and David Baunoch.

View on Journal Site

17

Peer-reviewed

Elevated UTI Biomarkers in Symptomatic Patients with Urine Microbial Densities of 10,000 CFU/mL Indicate a Lower Threshold for Diagnosing UTIs

Diagnostics · 16 August 2023

Laura K. S. Parnell, Natalie Luke, Mohit Mathur, Richard A. Festa, Emery Haley, Jimin Wang, Yan Jiang, Lori Anderson and David Baunoch.

View on Journal Site

18

Peer-reviewed

Comparison Shows that Multiplex Polymerase Chain Reaction Identifies Infection-associated Urinary Biomarker–positive Urinary Tract Infections That Are Missed by Standard Urine Culture

European Urology Open Science · December 2023

Emery Haley, Natalie Luke, Mohit Mathur, Richard A. Festa, Jimin Wang, Yan Jiang, Lori Anderson, David Baunoch

View on Journal Site

19

Peer-reviewed

A test combining multiplex-PCR with pooled antibiotic susceptibility testing has high correlation with expanded urine culture for detection of live bacteria in urine samples of suspected UTI patients

Diagnostic Microbiology and Infectious Disease · 3 July 2023

Richard A. Festa, Natalie Luke, Mohit Mathur, Laura Parnell, Dakun Wang, Xinhua Zhaob, Jesus Magallona, Marina Remedios-Chan, Jasmine Nguyen, Tim Cho, Annie Ngo, Max Murphy, and David Baunoch.

Read summary

20

Peer-reviewed

The Prevalence and Association of Different Uropathogens Detected by M-PCR with Infection-Associated Urine Biomarkers in Urinary Tract Infections

Research and Reports in Urology · 9 January 2024

Emery Haley, Natalie Luke, Mohit Mathur, Richard A. Festa, Jimin Wang, Yan Jiang, Lori Anderson, David Baunoch

View on Journal Site

21

Peer-reviewed

Emerging and Fastidious Uropathogens Were Detected by M-PCR with Similar Prevalence and Cell Density in Catheter and Midstream Voided Urine Indicating the Importance of These Microbes in Causing UTIs

Infection and Drug Resistance · 23 December 2023

Dakun Wang, Emery Haley, Natalie Luke, Mohit Mathur, Richard A Festa, Xinhua Zhao, Lori A Anderson, Jennifer L Allison, Kristen L Stebbins, Manuel Jose Diaz, David Baunoch

View on Journal Site

22

Peer-reviewed

Microbial Threshold Guidelines for UTI Diagnosis: A Scoping Systematic Review

Pathology and Laboratory Medicine International · 6 August 2023

Evann E Hilt, Laura KS Parnell, Dakun Wang, Ann E Stapleton, and Emily S Lukacz.

View on Journal Site

23

Peer-reviewed

After 180 Years, Is it Time for Something Better for Diagnosing UTIs?

JOJ Urology & Nephrology · 2 September 2020

Natalie Luke and David Baunoch.

Read summary

24

Peer-reviewed

Beyond the Usual Suspects: Emerging Uropathogens in The Microbiome Age

Frontiers in Urology · 26 July 2023

Robert B. Moreland, Brian I. Choi, Wilson Geaman, Caroline Gonzalez, Baylie R. Hochstedler-Kramer, Jerrin John, et al.

View on Journal Site

25

Peer-reviewed

Tarnished Gold—The “Standard” Urine Culture: Reassessing The Characteristics of a Criterion Standard for Detecting Urinary Microbes

Frontiers in Urology · 11 July 2023

Linda Brubaker, Toby C. Chai, Harry Horsley, Rajvinder Khasriya, Robert B. Moreland, and Alan J. Wolfe.

View on Journal Site

26

Peer-reviewed

Comparing Prescribing Behaviors and Clinician Experiences Between Multiplex PCR/Pooled Antibiotic Susceptibility Testing and Standard Urine Culture in Complicated UTI Cases

J Clin Med · 7 December 2024

Emery Haley, Natalie Luke, Howard Korman, Ganesh Srinvas Rao, David Baunoch, Xiaofei Chen, Jim Havrilla and Mohit Mathur

View on Journal Site

27

Peer-reviewed

From Awareness to Action: Pioneering Solutions for Women’s UTI Challenges in the Era of Precision Medicine

International Journal of Women’s Health · 28 September 2024

Emery Haley and Natalie Luke.

View on Journal Site

28

Peer-reviewed

Polymicrobial Urine Cultures: Reconciling Contamination with the Urobiome while Recognizing the Pathogens

Frontiers in Cellular and Infection Microbiology · 19 May 2025

Robert B. Moreland, Linda Brubaker and Alan J. Wolfe.

View on Journal Site

29

Editorial

Potential System Utilization and Cost Savings of Advanced Microbial Testing for Urinary Tract Infections

JU Open Plus · 26 May 2023

John Myrga, Christopher Staniorski and Bruce Jacobs.

View on Journal Site